Images

Images from the work of the C-TRACER team:

Figure 1: Picture of the culture plates shows monolayer of cultivated limbal epithelial cells on day 12 of culture.

Figure 2: This clinical picture is of a six year old patient with chemical burs(A); presentation four weeks after treatment with cultivated autologus limbal epithelials cells(B), 37 months after penetrating keratoplasty (corneal transplantation) (C). The light microscopy of excised cornea shows a normal looking stratified corneal epithelium adherent to the underlying corneal stroma. (D) (Hematoxylin and eosin x 125)

Figure 3: Laser Scanning Confocal Microscopic picture of section of corneal button obtained from patient undergoing cultivated limbal epithelial transplantation followed by penetrating keratoplasty showing positivity (green fluorescence) for corneal lineage specific cytokeratin K3 (Nuclei – Red, counterstained with propidium iodide)

Figure 4: Laser Scanning Confocal Microscopic picture of section of corneal button obtained from patient undergoing cultivated limbal epithelial transplantation followed by penetrating keratoplasty showing positivity for collagen IV (green fluorescence) indicating basement membrane formation (Nuclei – Red, counterstained with propidium iodide)